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SDS quantitation and removal with resins

SDS quantitation and removal with resins - Protocols and Methods Forum

SDS quantitation and removal with resins - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-02-2003, 02:03 PM
Nick Holway
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Default SDS quantitation and removal with resins



Scott Coutts <[Only registered users see links. ].au> wrote in message news:<[Only registered users see links. ].au>...

You could always attack the problem from a different angle and use the
SDS as a blank and then subtract its absorbance from the value with
the sample in it.

Nick
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Old 07-03-2003, 01:48 AM
Scott Coutts
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Default SDS quantitation and removal with resins



Nick Holway wrote:

Yup, but I dont want to assay for protien concentration! That would be
much easier.

There's an unknown SDS and unknown protein concentration in these
samples. Effectively, it's the SDS that IS the sample. That's why I
wanted to know if there's a way to assay for SDS itself.

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