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Positive pressure in lab

Positive pressure in lab - Protocols and Methods Forum

Positive pressure in lab - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-01-2006, 06:47 AM
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Default Positive pressure in lab



For cell culture lab it is usually required that we have an installation of Air handling unit to purify the air that circulates in the room, but that also create positive pressure in the room, and in the same cell culture room we have instruments like biosafty cabinet and laminar flower that also have positive pressure, so
due to presence of positive pressure in room and also within these instruments is it the possibiltiy of interfaring of working and also interfarence in the positive pressures, and the chances that air from the room with positive pressure distrub the positive pressure within the instruments mentioned above and result in contemination.

the person who have such an experience plz guide me.

aftab
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Old 07-13-2006, 02:21 PM
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Default Re: Positive pressure in lab

Aftab,

I have had experience with your situation. The air purification unit must be placed/installed away from the biosafety cabinets as far as is possible. The effects of the biosaftey cabinets are necessarily limited to the area within the cabinet itself, so this will not interfere with the room's air circulation. The effect of the air purifier although affecting the majority of the lab, will fall gradually with distance from the unit itself. We had the same dilemma when we were fitting out unit and we were advised to place it in the opposite corner of the room from the biosafety cabinets.

Hope this helps,

Canalba, UK
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Old 08-21-2006, 03:49 PM
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Default Re: Positive pressure in lab

what should the pressure be in w.c.?
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Old 08-22-2006, 03:22 PM
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Default Re: Positive pressure in lab

Great post Canalba,
Jamey i am not sure I will check though
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