promoter+reporter/transactivator ratio???

[venusia]

Hello to everybody!

I am doing transactivation experiments to analyse a promoter for the first time, and I have some questions.
1) In the beginning I have tried three promoter+reporter/transactivator ratio: 1:1; 1:2; and 1:3.
Of course all was done in triplicate including a negative control.
Everything worked fine as I had increasing fold over control values: 10 in 1:1, 15 in 1:2 and 26 in 1:3.

Now my question is: if I want to publish these data, which of the three experiments shoud I use? Of course I would like to use the 1:3 ones which give a higher transactivation values but I have been told that this means "cheating" and that the only set I can use is the 1:1.
On the other hand other people agree with the 1:3.

This subject is new for me, so I would like to have a more general and international opinion.

Than you very much, you help is VERY precious to me!