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2D gel running problem

2D gel running problem - Protocols and Methods Forum

2D gel running problem - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-21-2007, 10:08 AM
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Unhappy 2D gel running problem



Hi I've been having a certain problem when running my 11cm 2D gels in a Criterion gel tank by Bio-Rad. I run them under constant voltage conditions of 200V but I seem to get really low mA values. As a result my gels will either not run at all giving me a total smear when stained or stop running midway through. Additionally it takes 2-3 hours for them yo run as opposed to 50 min previously. Lately I have been having the same problem with my 1D gels as well. From that I thought that the problem was with my electrical source ( PowerPac 300) but I switched it with another one that we had in the lab but still have the same problem. Any ideas on what maybe at fault or what to do?

Thanks,
Chris
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