Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Protocols and Methods Forum (http://www.molecularstation.com/forum/protocols-methods-forum/)
-   -   2D gel running problem (http://www.molecularstation.com/forum/protocols-methods-forum/1007-2d-gel-running-problem.html)

cosmis85 02-21-2007 10:08 AM

2D gel running problem
 
Hi I've been having a certain problem when running my 11cm 2D gels in a Criterion gel tank by Bio-Rad. I run them under constant voltage conditions of 200V but I seem to get really low mA values. As a result my gels will either not run at all giving me a total smear when stained or stop running midway through. Additionally it takes 2-3 hours for them yo run as opposed to 50 min previously. Lately I have been having the same problem with my 1D gels as well. From that I thought that the problem was with my electrical source ( PowerPac 300) but I switched it with another one that we had in the lab but still have the same problem. Any ideas on what maybe at fault or what to do?

Thanks,
Chris


All times are GMT. The time now is 06:59 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08820 seconds with 11 queries