Spot analysis

[janemon]

Hi all,
I'm analyzing spots using software. The spots with high intensity are fainted and the software detects them as the low expression proteins. Can anyone please tell me on how to fix this problem?
Thank you.

[cattoyee]

Hi,
Maybe you should try analyse the volume percentage instead of intensity because there would be a lot of variable if you use intensity to analyse the spots. Just an idea.
Regards...cattoyee

[protdoc]

yes cattoyee is right... analyse the spot volume rather than intensity

[danfive]

What software are you using?

[janemon]

I' m using PDquest. Thank you.

[milestone]

I am using PDquest ,also. I think maybe you should chek the background colour ro noise which can influence the analysis becaues the soft can not distinguish the spot and noise. Secondly ,you should read the protocol of this soft.Generally, the spot must be normalized before the quantitive compare.

[danfive]

Cold spring harbor has a great training protocol for pdquest [Only registered users see links. ]
If you did the online training webinar, module 6-8 should look familiar.