PROTEOMICS confusion? Confused about this analysis. do you
label with tags first to the n terminal residues?
or do digestion of proteins into peptides first and then label?
what is fractionation? is it the fragmentation of the tag into fragments? and if so what does this allow us to measure?
if tags are isobaric (same mass) but fragments how can you quantitate the protein?
MASCOT a database, is used to compare the fragmented pattern to idetify the labelled peptides? how does this work if the tag has been fragmented?
what is meant by "mass reporter ion??
im so confused with this technique PLEASE DO NOT LINK ME TO WIKIPEDIA. i have tried this already