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(iTRAQ analysis) isobaric tag for relative and absolute quantitation-

(iTRAQ analysis) isobaric tag for relative and absolute quantitation- - Proteomics Forum

(iTRAQ analysis) isobaric tag for relative and absolute quantitation- - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 01-14-2014, 04:26 PM
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Default (iTRAQ analysis) isobaric tag for relative and absolute quantitation-



PROTEOMICS confusion? Confused about this analysis. do you

label with tags first to the n terminal residues?
or do digestion of proteins into peptides first and then label?

what is fractionation? is it the fragmentation of the tag into fragments? and if so what does this allow us to measure?

if tags are isobaric (same mass) but fragments how can you quantitate the protein?

MASCOT a database, is used to compare the fragmented pattern to idetify the labelled peptides? how does this work if the tag has been fragmented?

what is meant by "mass reporter ion??



im so confused with this technique PLEASE DO NOT LINK ME TO WIKIPEDIA. i have tried this already
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