IEF - Isoelectric Focusing

Jules · #1 (**permalink**) 06-07-2006, 07:10 AM

I have a questions about IEF - Isoelectric Focusing

how long and what voltage do you do when using drystrips Amersham GE Sensing?

Blottingfreak · #2 (**permalink**) 07-05-2006, 05:52 PM

Isoelectric focusing on drystrips Amersham GE Sensing is conducted stepwise.
We run ours with the voltage increased to 8000V and focus for a total of 65000V/hr

for example take a look here:
http://www.weihenstephan.de/blm/deg/...ml02testp4.htm

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Member Login

IEF - Isoelectric Focusing

Proteomics Forum

Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.