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Membrane protein, detegents and MS detection

Membrane protein, detegents and MS detection - Proteomics Forum

Membrane protein, detegents and MS detection - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 07-09-2010, 05:57 AM
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Default Membrane protein, detegents and MS detection



Hello,
What are the best detergents to use to solublize membrane proteins prior to LC-MS/MS analysis? does anyone used: RapiGest, CHAPS, and n-Dodecyl--maltoside and know what is the best for solubization and does not suppress the signal and also does not affect trypsin? In case of CHAPS, and n-Dodecyl--maltoside at what %ACN are they out of the column? Does anyone know a good publication that compare different surfactants?
Thank you for the help
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Old 07-09-2010, 05:38 PM
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Default Re: Membrane protein, detegents and MS detection

GuHCl and Chaps work very well.
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