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Differences between precast- and lab-made gels??!

Differences between precast- and lab-made gels??! - Proteomics Forum

Differences between precast- and lab-made gels??! - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 04-02-2010, 05:23 PM
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Default Differences between precast- and lab-made gels??!



Hello!
Due to poor quality of precast gels for second dimension we chose to use lab-made gels. Despite the same degree of cross-linking of the both there a severe differences in spot patterns, such as a proper spot matching is virtually not possible. On the other hand, the gels within the groups are consistent. Did you notice similar problems and how did you solve them- without repeating the gels (which is no option because of no material left for any further processing).

Thank you for your help.
Happy Easter!
A.
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