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Lysine acetylation and tryptic digestion

Lysine acetylation and tryptic digestion - Proteomics Forum

Lysine acetylation and tryptic digestion - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 01-30-2010, 05:46 PM
Pipette Filler
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Default Lysine acetylation and tryptic digestion



Hello everyone--

I have a large synthetic peptide (>8 kDa) I wish to analyze by MS (MALDI). However, getting in-tact MW for this peptide is hopeless, as our instrument sensitivity falls off dramatically > 3 kDa

I'm considering a tryptic digest. If LYS residues are rendered unrecognizable by trypsin, hydrolysis only at ARG residues will yield 4 fragments of ideal size for detection.

Is acetylation with acetic anhydride the best option for modifying lys?

I have read that free cys can also become acetylated during this process, so preliminary carboxymethylation of cys was recommended. Is this so?
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Old 06-24-2010, 12:35 PM
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Default Re: Lysine acetylation and tryptic digestion

You can use an ArgC protease. Consider other chemicals and proteases as well

If you don't have these proteases/chemicals at your disposal acetylation is probably the best this to do. You will have to do carboxymethylation anyway for MS (it is a general practise)
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Old 07-20-2011, 06:34 AM
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Default Re: Lysine acetylation and tryptic digestion

Chemical acetylation can be messy. I would not recommend it. I like the idea of using a different enzyme as well. ArgC seems like the obvious choice. Much better than trying to modify Ks and using trypsin.
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Old 11-04-2011, 02:27 PM
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Default Re: Lysine acetylation and tryptic digestion

nice no doubt..
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acetylation , digestion , lysine , tryptic


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