| | Re: IP quantification
The WB can be used to predict concentrations of unknowns/samples as well.
I use Quantity One software--I set up stds in lanes (~3; high, med, low conc'ns)--and the software allows me to circle the bands and input the known concentrations. Then, voila, I have a linear std curve. That then predicts the conc. of unknown samples.
What it does is measure intensity/mm^2.
Of course I have to check the accuracy of this software:
on gel bands (coomasie blue stain)--accuracy, measured in % error 3.4-11.25%
on Western Blot (luminiscent signal)--accuracy, measured in % error 4.67-13.1%
Not too, shabby.