| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| Proteomics Forum Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc. |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
11-28-2006, 08:19 PM
| ||||||
| ||||||
 2-D Gel Problems with IEF Hey guys, I am having problems with my IEF (isoelectric focusing). It seems the second dimension is fine, but the IEF is messing up somehow. I think it may be the voltages. Does anyone have any recommendations for the voltages? thanks Kiki        |
| Today
| ||||
| ||||
 Sponsored Links |
|
03-26-2007, 11:02 PM
| |||||
| |||||
 Re: 2-D Gel Problems with IEF Using Biorad Ready Gels I use the following settings: 100V 60 min 250V 60 min 500V 60 min 25mA Max 10W I run the gels using NaOH in the inner chamber and Phosphoric acid in the outer chamber. Works well for me. |
|
05-29-2007, 01:27 AM
| |||||
| |||||
| Re: 2-D Gel Problems with IEF Hi Whts the programme you gave for IEF and wht type of fluctations you notice. write in detail regards SIvaramaiah |
|
| Tags |
| gel , ief , problems |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Problems with Invitrogen? | steveF | Bad Product/Service? Post Here | 0 | 11-15-2007 04:38 PM |
| IEF problems | rkwi2007 | Proteomics Forum | 1 | 07-17-2007 07:56 PM |
| Problems with 2d minigels | giasco78 | Proteomics Forum | 4 | 06-04-2007 09:50 AM |
| Molecular Station Site Down Due to Server Problems | admin | Molecular Biology News and Announcements | 3 | 11-30-2006 08:18 PM |
| Inducible deletion problems...please help! | dedrical | Knockout Mouse Forum | 1 | 09-18-2006 08:15 AM |
Linear Mode
