| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Proteomics Forum Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| Hi guys I Just wanted to try get a general conscious on what is the best approach I should take. I’m undertaking an industrial PhD were I am identifying novel enzymes, over the past year I have been quite successful and been able to purify and identify my protein using Maldi (as a related genome had already been sequenced it was relatively easy) I am about to start work on a new enzyme from a different organism, as my work is part of a bigger project there is generally a strong emphasis on indentifying the protein ASAP so that I can work on making this enzyme a product. If I go down the same route I doubt I will be able to identify the protein by tryptic digestion (no similar genomes have been sequenced) As far as I can figure out I have four options: *Maldi -Post source decay sequencing, but been told very time consuming *Send away for N-terminal sequencing then do degenerate PCR, but I believe this approach can be a bit hit a miss. *Edman sequencing (limited to short aa lengths but can be done if protein digested, does anyone do a full protein service?) *Get entire bacteria genome sequenced (I know it sounds daft but have read prices dropped dramatically to around US $1000 ) that way I could just do the relatively easy tryptic digest and use Maldi. Given I need results ASAP (cant spend over a year trying to find the sequence of my protein) what approach would you guys take? Are there any other ways I could identify my protein sequence? |
| Tags |
| protein |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Human Cytome Project - an idea - Update 19 April 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 06-01-2009 02:17 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
| New Saccharomyces Sequences 11/27/04 | Mike Cherry | Yeast Forum | 0 | 11-28-2004 11:39 PM |
| New Saccharomyces Sequences 05/19/04 | SGD Sequences | Yeast Forum | 0 | 05-23-2004 04:06 PM |