I need Help!
Heres my problem hope someone can help me out!
I have several cDNA sequences after differential display rt-pcr. With one cDNA sequence I was able to purify it and clone it into TA plasmids then sequence. When I BLASTn with any existing database on C. albicans no significant similarity could be found to any gene, in fact, no C. albicans identity at all! The BLASTn result gave low max identity to other organisms. Even BLASTx.
Does this mean my cDNA is not from C albicans at all? (But BLAST genome gave whole-genome shotgun sequence for some of my unidentified cDNAs).If there's really no BLAST identity is it a novel gene?
Since the C. albicans genome has been sequenced, it is even possible that this happens? Is my cDNA finding anything significant? What can I do next? Use the cDNA as labeled probe to the CA genome library?
I need advice! Any help please post!!