2-D gel staining

[deebee12]

I have a question on 2-D gel staining. What is the best stain to use I am confused?

- Silver stain?
- Coomasie?
- Sypro Ruby?
- Other?

[joey20]

It really depends what you are staining for and how abundant (how much protein) your protein is.

I would first try silver staining. Just make sure you silver stain with a stain you can send for sequencing. SYPRO ruby is great also if you have it although you need UV light to detect your bands.

You need TONS of protein for COOMASIE.

[sinapis]

Hello, I use COOMASIE and I use only 5 micrograms, so it is not so much.

[leaf]

the silver staining is more sensitive,but it also has higher background than Coomassie.And the SYPRO ruby is well and can be compatible with the mass spectrometry.

[danfive]

Imperial Protein Stain from Pierce--no toxic chemicals--no fluourescence light needed to visualize--and only slightly less sensitive than Sypro Ruby. Very stable, no background problems, also completely compatible with MS.