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extraction buffer solidifies on ice

extraction buffer solidifies on ice - Proteomics Forum

extraction buffer solidifies on ice - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 12-03-2007, 08:28 AM
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Default extraction buffer solidifies on ice



hello
i prepare extraction buffer for proteins and when i keep it on ice it solidified,i think urea crystalizes but before this it never happened.i am using 8M urea.
what could be the reason
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Old 12-03-2007, 06:09 PM
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Default Re: extraction buffer solidifies on ice

You're right, it crystallizes at low temperatures. Use Urea at or near room temperature.
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