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-   -   extraction buffer solidifies on ice (http://www.molecularstation.com/forum/proteomics-forum/2545-extraction-buffer-solidifies-ice.html)

irfan_sadiq 12-03-2007 08:28 AM

extraction buffer solidifies on ice
 
hello
i prepare extraction buffer for proteins and when i keep it on ice it solidified,i think urea crystalizes but before this it never happened.i am using 8M urea.
what could be the reason

danfive 12-03-2007 06:09 PM

Re: extraction buffer solidifies on ice
 
You're right, it crystallizes at low temperatures. Use Urea at or near room temperature.


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