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no protein spots in acidic range

no protein spots in acidic range - Proteomics Forum

no protein spots in acidic range - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 11-27-2007, 09:55 AM
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Default no protein spots in acidic range



i am running proteins of arabidopsis.i am using ph range 3-10 there are no protein spots in acidic range.
what it could be the reason?
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Old 11-29-2007, 01:15 PM
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Default Re: no protein spots in acidic range

Is there no spot at all?
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Old 11-30-2007, 08:34 AM
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Default Re: no protein spots in acidic range

no,there is no spot at all
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Old 12-03-2007, 08:05 AM
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Default Re: no protein spots in acidic range

Sounds like an IEF problem to me, what's the pH of your sample before IEF, do you add enough DTT and stuff like that before IEF?
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Old 12-03-2007, 10:55 AM
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Default Re: no protein spots in acidic range

Are you running total protein? Maybe there just are no acidic proteins, or they are in very low concentration. I've run trypanosome total proteins using 3-10 grad and there are very few (3 or 4) spots on the acidic side.
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