I found quite a few more as well thanks Danfive.
A few more causes and solutions for Horizontal 2-D gel streaking:
your sample was not completely solubilized prior to 2-D gel loading
solution: Make sure that your sample is completely and stably solubilized your sample was poorly soluble in the rehydration solution
solution: Increase the concentration of the solubilizing components in the rehydration buffer and/or Increase the concentration of IPG buffer. your sample has Interfering Substances or Impurities.
solution: Modify or Purify sample preparation to limit contamination of these interfering substances your sample has High Salt or Ionic Impurities
solution: you can reduce the salt concentration below 10mM by dilution or dialysis of your samples.
Also some more:
- If you used SDS which is an ionic detergent, it must not exceed 0.25%
- You can also try loading less sample.
- Prolong the focusing time and maybe reduce it also.
Please tell us what works
may help us all in the future!