Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Proteomics Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

Proteomics Forum Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


Problems with protein quantification

Proteomics Forum

Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 09-06-2007, 10:19 AM
Pipette Filler
Points: 1,089, Level: 18Points: 1,089, Level: 18Points: 1,089, Level: 18
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Oct 2006
Posts: 3
Amiodaron RSS Feed
Default Problems with protein quantification

Hello!
For protein quantification I wanted to use the 2D-Quant Kit (GE), but had some problems with it. There is one thing I really cannot explain: How can I get several totally different protein concentrations by using just different dilutions of my protein solution?? Does anyone exist who also uses this kit? Or what`s your favoured method for protein quantification?
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

  #2 (permalink)  
Old 09-06-2007, 03:00 PM
danfive's Avatar
M.D/Ph.D
Points: 2,193, Level: 29Points: 2,193, Level: 29Points: 2,193, Level: 29
Activity: 100%Activity: 100%Activity: 100%
 

Join Date: Jul 2007
Location: Houston TX
Posts: 491
danfive RSS Feed
Default Re: Problems with protein quantification

You can get different concentrations if you have a buffer reagent that interferes with the kit's reagents. A lot of salts and detergents can be diluted out so they don't interfere with the quantitation reaction, so they have their cutoff limits of what concentration or percent will work with the kit. Check your buffer recipes and kit manual for any interfering agents that may be in the protein solution.
I use microBCA kit from Pierce.
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Bradford assay - protein quantification using BSA Standard. but i encounter problems foRk Protocols and Methods Forum 0 04-24-2008 01:21 AM
Protein Extraction Problems molecule2005 Protein Science 0 04-16-2008 08:57 AM
His Tag Protein Purification Problems IoDiNE Recombinant Protein Forum 1 02-26-2008 02:22 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 1 02-17-2008 02:00 PM
Protein Quantification sazza7 Protein Science 1 11-04-2007 06:29 PM


All times are GMT. The time now is 03:25 AM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved