unusual result of protein running in the SDS gel

[Maniche]

Hey All,

I am used to running a protein extract in an SDS gel at 4 degree celsius and used to get a high molecular protein band.

however i started running the same protein extract in a similar gel but at room temperature and didnt see any high molecular band.Although i could see a new band at a low molecular size.

Does anyone have come across such a case wherein, a high molecular wt protein band has moved down to a low molecular wt band just becoz of running of gel at room temperature?????

[benjamin]

The proteins could have degraded in the room temp. you should try to standardize the running conditions.