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peptide extraction question

peptide extraction question - Proteomics Forum

peptide extraction question - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 09-01-2007, 04:56 PM
Pipette Filler
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Question peptide extraction question



Hello,
Can someone please answer me this:

In a certain procedure, proteins that were in gel slices went through destain, reduction, alkylation, and typsin digestion. Then peptides were extracted 3 times using just 50mM ammonium bicarbonate placed in a 37*C incubator, with no organic solvents being added (i.e: 10% ACN or 50% ACN).

Will ANY peptides extract from this procedure? And if so, how much generally speaking? Any information will be helpful!
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Old 01-10-2008, 01:10 AM
Pipette Filler
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Default Re: peptide extraction question

you should add ACN
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