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09-01-2007, 04:56 PM
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 peptide extraction question Hello, Can someone please answer me this: In a certain procedure, proteins that were in gel slices went through destain, reduction, alkylation, and typsin digestion. Then peptides were extracted 3 times using just 50mM ammonium bicarbonate placed in a 37*C incubator, with no organic solvents being added (i.e: 10% ACN or 50% ACN). Will ANY peptides extract from this procedure? And if so, how much generally speaking? Any information will be helpful!        |
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Re: peptide extraction question 
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