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-   -   peptide extraction question (http://www.molecularstation.com/forum/proteomics-forum/1967-peptide-extraction-question.html)

molbio123 09-01-2007 04:56 PM

peptide extraction question
 
Hello,
Can someone please answer me this:

In a certain procedure, proteins that were in gel slices went through destain, reduction, alkylation, and typsin digestion. Then peptides were extracted 3 times using just 50mM ammonium bicarbonate placed in a 37*C incubator, with no organic solvents being added (i.e: 10% ACN or 50% ACN).

Will ANY peptides extract from this procedure? And if so, how much generally speaking? Any information will be helpful!

davistone 01-10-2008 01:10 AM

Re: peptide extraction question
 
you should add ACN


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