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2-D Gel Electrophoresis Phosphorylation Analysis
I am conducting 2-D gel electrophoresis for phosphorylated protein analysis.
The problem is there are tons of dephosphorylating enzymes in my mixture such as phosphatases.
How can I reliably inhibit these guys? What phosphatase inhibitors are you guys using?
Tyrosine phosphatase inhibitors? Serine phosphatase inhibitors?
Re: 2-D Gel Electrophoresis Phosphorylation Analysis
I use ortho vanadate (10mM I think) for protein phosphotyrosyl phosphatases.
|analysis , electrophoresis , gel , phosphorylation|
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