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2-D Gel Electrophoresis Phosphorylation Analysis

2-D Gel Electrophoresis Phosphorylation Analysis - Proteomics Forum

2-D Gel Electrophoresis Phosphorylation Analysis - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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Old 08-27-2007, 12:14 PM
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Cool 2-D Gel Electrophoresis Phosphorylation Analysis



Hello everyone,

I am conducting 2-D gel electrophoresis for phosphorylated protein analysis.

The problem is there are tons of dephosphorylating enzymes in my mixture such as phosphatases.

How can I reliably inhibit these guys? What phosphatase inhibitors are you guys using?

Tyrosine phosphatase inhibitors? Serine phosphatase inhibitors?

Thanks!
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Old 08-27-2007, 12:14 PM
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Smile Re: 2-D Gel Electrophoresis Phosphorylation Analysis

I use ortho vanadate (10mM I think) for protein phosphotyrosyl phosphatases.
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