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| For Background--not smearing, dropping out, smudging--washing your gel before staining helps a great deal. You can rinse and wash for 30 minutes to 1 hour--I suggest rinsing 4 to 5 times in 4X volume--then washing on a shaker gently for 30-60 minutes--then using a minimal amount of time for staining. Before visualization rinse again. Also use new--not reused staining solution. Some people rinse and then fix gel with a TCA solution--not good for MS downstreaming though---I find this to be overkill. |
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