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Streaking of 2-D SDS-Gel Electrophoresis

Streaking of 2-D SDS-Gel Electrophoresis - Proteomics Forum

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  #1  
Old 07-05-2006, 05:54 PM
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Question Streaking of 2-D SDS-Gel Electrophoresis



I am getting streak of my 2-D SDS-Gel.

is it because I am loading too much protein or because the focusing is not working?
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  #2  
Old 07-05-2006, 05:57 PM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

Are you getting vertical or horizontal streaking?

see this information on 2D Streaking and solutions:

Troubleshooting 2D Gels
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Old 07-13-2006, 11:50 AM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

I Want To Know The Cause Too.
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Old 08-01-2006, 03:40 PM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

could pls explain wether ur getttin vertical or horizontal streaks?
if possible u can load the 2D image so that we can discuss more
shivram.N
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Old 08-01-2006, 03:41 PM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

Hi
cananyone help me in analysis of PD QUEST 2D software particularly with stastical analysis
shivram.N
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Old 08-02-2006, 01:36 AM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

Are you loading too much protein?

Have you checked your buffers? for IEF? for the gel?
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Old 09-19-2006, 08:47 PM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

most likely something wrong with first dimension IEF.
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Old 09-20-2006, 06:13 AM
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Default Re: Streaking of 2-D SDS-Gel Electrophoresis

Yeah good idea...
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