2D gel running problem

cosmis85 · #1 (**permalink**) 02-22-2007, 10:36 AM

Hi I've been having a certain problem when running my 11cm 2D gels in a Criterion gel tank by Bio-Rad. I run them under constant voltage conditions of 200V but I seem to get really low mA values. As a result my gels will either not run at all giving me a total smear when stained or stop running midway through. Additionally it takes 2-3 hours for them yo run as opposed to 50 min previously. Lately I have been having the same problem with my 1D gels as well. From that I thought that the problem was with my electrical source ( PowerPac 300) but I switched it with another one that we had in the lab but still have the same problem. Any ideas on what maybe at fault or what to do?

apsbabu · #2 (**permalink**) 03-07-2007, 02:01 AM

Check your Buffer .. I think there is too little salt in it !

leaf · #3 (**permalink**) 05-25-2007, 06:41 AM

I think the problem lies in you buffer,prepare a new buffer and do it again.

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