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2D gel running problem

2D gel running problem - Proteomics Forum

2D gel running problem - Post Questions and Discuss Proteomics, Proteomic Bioinformatics, Proteomic Techniques such as 2-D, Mass Spec etc.


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  #1  
Old 02-22-2007, 11:36 AM
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Unhappy 2D gel running problem



Hi I've been having a certain problem when running my 11cm 2D gels in a Criterion gel tank by Bio-Rad. I run them under constant voltage conditions of 200V but I seem to get really low mA values. As a result my gels will either not run at all giving me a total smear when stained or stop running midway through. Additionally it takes 2-3 hours for them yo run as opposed to 50 min previously. Lately I have been having the same problem with my 1D gels as well. From that I thought that the problem was with my electrical source ( PowerPac 300) but I switched it with another one that we had in the lab but still have the same problem. Any ideas on what maybe at fault or what to do?
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Old 03-07-2007, 03:01 AM
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Default Re: 2D gel running problem

Check your Buffer .. I think there is too little salt in it !
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Old 05-25-2007, 06:41 AM
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Default Re: 2D gel running problem

I think the problem lies in you buffer,prepare a new buffer and do it again.
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Old 09-12-2009, 08:48 PM
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Default Re: 2D gel running problem

The first problem is usually the powersupply however you should try a ready made buffer to eliminate buffer problems. If the problems still exist try running your proteins on a different system. I highly reccomend the Gebagel System from chembio Ltd.

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Old 12-31-2009, 05:26 AM
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Default Re: 2D gel running problem

Do you prepare your gel correctly? Check if you use old acrylamide. Make sure you never recycled your buffer, use fresh for every run.
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