Originally Posted by proteoglycan
I tried dialysis and lost approx. 3/4 of my starting protein concentration (after purification).
Hi! As I truly understand firstly you made purification under denaturating conditions in 6M urea. So afer purification you made dialysis and lost your protein. So what Mw of your protein and Mw cuttoff of your membrane? The really Mw of denaturated proteins are smaller then native. To avoid loss of your protein use Dialysis tube with smaller cutoff/ For ex when i dialysed my protein with Mw 24kDa I used DT cutoff 8kDa. If I used DT cutoff 12kDa I lose my protein
If your add acetic acid to buffer of your composition I think you do not make it volatile Because as i know only ammonium acetate is volatile! I don't understand your strategy if your use spec measurment at 400nM