WB of low MW peptides- optimization?

amessusa · #1 (**permalink**) 02-15-2007, 06:00 PM

I work with rat brain studying ageing. My WB's with peptides of moderate size (40-140 kDa) are OK but I would like to see if someone can help me with determining a Beta peptides 1-40 and 1-42. Their MW are in the range of 4-6 kDa. I have seen references using 15 or 18 % gels. When I run an 18% gel the MW standards and the target peptides are not optimal. Thanks

kmunson779 · #2 (**permalink**) 02-15-2007, 06:26 PM

Have you tried tris-tricine gels?

sergbio · #3 (**permalink**) 02-18-2007, 07:54 PM

16 OR 20% Tris-tricine PAGE work good!

amessusa · #4 (**permalink**) 02-19-2007, 01:46 PM

kmunson and sergbio, Thank you- I will try these.

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WB of low MW peptides- optimization?

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