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How to determine the binding number of Alkaline Phosphatase on Streptavidin during th

How to determine the binding number of Alkaline Phosphatase on Streptavidin during th - Protein Science

How to determine the binding number of Alkaline Phosphatase on Streptavidin during th - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 07-21-2014, 07:53 AM
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Smile How to determine the binding number of Alkaline Phosphatase on Streptavidin during th



Dear all,

I want to congugate antibody with Alkaline Phosphatase. Is there any way that I can determine the antibody to AP ratio? (Alkaline Phosphatase : 140-160 kDA, Antibody : ~150 kDA) Thank you very much for your answer in advance.

Sincerely,
Adrian
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