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Weird problem in SDS_PAGE

Weird problem in SDS_PAGE - Protein Science

Weird problem in SDS_PAGE - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 11-21-2012, 01:30 AM
Pipette Filler
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Default Weird problem in SDS_PAGE



Hello.

I am doing some SDS_PAGE for rhFSH. I liophilized some samples of pure FSH that I used ir order to evaluate some cromatographical matixes.
But some samples were weird when I was going to use them for the PAGE. I mixed them with loading buffer but 3 samples instead of turning blue get some strange light blue/incolour. Once I staing my gel with Coomasie solution I get this: as u can see in the pictures there are some strong blue weird camel humps.

I would like to know if someone has an idea about that.

Thank you very much!

Greetings from Argentina!
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Old 11-27-2012, 04:45 PM
Pipette Filler
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Default Re: Weird problem in SDS_PAGE

so nobody has any clue about this problem?
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