Weird problem in SDS_PAGE
 

Hello.

I am doing some SDS_PAGE for rhFSH. I liophilized some samples of pure FSH that I used ir order to evaluate some cromatographical matixes.
But some samples were weird when I was going to use them for the PAGE. I mixed them with loading buffer but 3 samples instead of turning blue get some strange light blue/incolour. Once I staing my gel with Coomasie solution I get this: as u can see in the pictures there are some strong blue weird camel humps.

I would like to know if someone has an idea about that.

Thank you very much!

Greetings from Argentina!

Re: Weird problem in SDS_PAGE
 

so nobody has any clue about this problem?