Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Protein Science (http://www.molecularstation.com/forum/protein-science/)
-   -   Weird problem in SDS_PAGE (http://www.molecularstation.com/forum/protein-science/86840-weird-problem-sds_page.html)

jgure 11-21-2012 01:30 AM

Weird problem in SDS_PAGE
 
2 Attachment(s)
Hello.

I am doing some SDS_PAGE for rhFSH. I liophilized some samples of pure FSH that I used ir order to evaluate some cromatographical matixes.
But some samples were weird when I was going to use them for the PAGE. I mixed them with loading buffer but 3 samples instead of turning blue get some strange light blue/incolour. Once I staing my gel with Coomasie solution I get this: as u can see in the pictures there are some strong blue weird camel humps.

I would like to know if someone has an idea about that.

Thank you very much!

Greetings from Argentina!

jgure 11-27-2012 04:45 PM

Re: Weird problem in SDS_PAGE
 
so nobody has any clue about this problem?


All times are GMT. The time now is 12:29 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08915 seconds with 11 queries