| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
01-25-2007, 08:26 AM
| |||||||||||
| |||||||||||
 recombinanat protein expression hi, i found some problem in expressing my protein in BL21 DE3 host. NO expression in the colony screening. my protein is also non toxic to the host. In the first day iam finding some very minimal expression, next day no expression. IPTG concentration is 1mM. vector i use is pRSETB. kndly guide me in this regard.  |
|
#2
01-26-2007, 01:12 AM
| ||||
| ||||
| Re: recombinanat protein expression Hello Gajji81! very tough observations. Not sure what you could do. Does your vector have high expression (with control protein) in BL21 DE3? welcome to the forums by the way!  |
|
#3
02-09-2007, 02:27 PM
| |||||||||||
| |||||||||||
| Re: recombinanat protein expression Hi Gajji81, I also have same problem in the protein expression in BL-21 (DE3). I have used the pRSETA, also no or little expression after IPTG induction. Have you already solved the problem, pls. let me know. Thanks. Yoki |
|
#6
10-15-2008, 01:42 PM
| |||||||||||
| |||||||||||
| Re: recombinanat protein expression How do you resolve that problem? |
|
| Tags |
| expression , protein , recombinanat |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Human Cytome Project - an idea - Update 19 April 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 06-01-2009 02:17 PM |
| A Human Cytome Project - an idea - Update 14 March 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 03-14-2005 01:27 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
| New Saccharomyces Sequences 05/19/04 | SGD Sequences | Yeast Forum | 0 | 05-23-2004 04:06 PM |
Linear Mode
