recombinanat protein expression

gajji81 · #1 (**permalink**) 01-25-2007, 08:26 AM

hi,

i found some problem in expressing my protein in BL21 DE3 host. NO expression in the colony screening. my protein is also non toxic to the host. In the first day iam finding some very minimal expression, next day no expression. IPTG concentration is 1mM. vector i use is pRSETB. kndly guide me in this regard.

moleculardude · #2 (**permalink**) 01-26-2007, 01:12 AM

Hello Gajji81!

very tough observations. Not sure what you could do. Does your vector have high expression (with control protein) in BL21 DE3?

welcome to the forums by the way!

yokibutt · #3 (**permalink**) 02-09-2007, 02:27 PM

Hi Gajji81,

I also have same problem in the protein expression in BL-21 (DE3). I have used the pRSETA, also no or little expression after IPTG induction. Have you already solved the problem, pls. let me know.
Thanks.
Yoki

ghazanfar · #4 (**permalink**) 02-21-2007, 11:57 AM

please supply me the informations about recombinant protien and its purification

ghazanfar · #5 (**permalink**) 02-21-2007, 12:01 PM

[quote=ghazanfar;2344]please supply me the informations about recombinant protien and its purification

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recombinanat protein expression

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