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recombinanat protein expression

recombinanat protein expression - Protein Science

recombinanat protein expression - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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  #1  
Old 01-25-2007, 09:26 AM
Pipette Filler
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Default recombinanat protein expression



hi,

i found some problem in expressing my protein in BL21 DE3 host. NO expression in the colony screening. my protein is also non toxic to the host. In the first day iam finding some very minimal expression, next day no expression. IPTG concentration is 1mM. vector i use is pRSETB. kndly guide me in this regard.
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Old 01-26-2007, 02:12 AM
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Default Re: recombinanat protein expression

Hello Gajji81!

very tough observations. Not sure what you could do. Does your vector have high expression (with control protein) in BL21 DE3?

welcome to the forums by the way!
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Old 02-09-2007, 03:27 PM
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Default Re: recombinanat protein expression

Hi Gajji81,

I also have same problem in the protein expression in BL-21 (DE3). I have used the pRSETA, also no or little expression after IPTG induction. Have you already solved the problem, pls. let me know.
Thanks.
Yoki
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Old 02-21-2007, 12:57 PM
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Default recombinanat protein purification method

please supply me the informations about recombinant protien and its purification
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Old 02-21-2007, 01:01 PM
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Default Re: recombinanat protein purification method

[QUOTE=ghazanfar;2344]please supply me the informations about recombinant protien and its purification
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Old 10-15-2008, 01:42 PM
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Default Re: recombinanat protein expression

How do you resolve that problem?
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