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ELISA v.s. Western blotting

ELISA v.s. Western blotting - Protein Science

ELISA v.s. Western blotting - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 05-15-2012, 09:54 AM
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Default ELISA v.s. Western blotting



Hi,

I'm having a bit of a problem in my research. I want to know in which organs my protein binds in (will be given via IV bolus dose) but I can't find any articles on wether ELISA or Western blotting suits me better.

I just need to know if the protein is there or not, quantification isn't necessary since I'll be doing cryohistology on the organs the protein is detected in. And I don't have enough time nor antibody to do both ELISA and western.

Any input would be wonderful!
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blotting , elisa , organ , protein , western , western blotting


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