Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


SDS PAGE spilling effect of protein

SDS PAGE spilling effect of protein - Protein Science

SDS PAGE spilling effect of protein - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-24-2011, 04:54 AM
Pipette Filler
Points: 2,397, Level: 31 Points: 2,397, Level: 31 Points: 2,397, Level: 31
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2008
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default SDS PAGE spilling effect of protein



Hello All,

We are facing a peculiar problem.

I have attached my SDS PAGE gel for reference. We get a pattern as if protein has spilled into adjacent well and the blank well (1st and last) also shows band. we have been performing the same method for same protein for about 7 yrs with no difficulty but from past 2-3 months are encountering this problem. we have tried preparing fresh reagents and do the test but same results. what can be the problem as while loading the protein does not spill, still this results is confusing.

Can anyone throw some light or elaborate on this. Any help will be appreciated.

Thanks in anticipation
Attached Images
File Type: jpg SDS GEL.jpg (42.4 KB, 11 views)
Reply With Quote
Reply

Tags
effect , page , protein , sds , spilling


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 04:57 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.18900 seconds with 17 queries