Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


sedimentation equilibrium help

sedimentation equilibrium help - Protein Science

sedimentation equilibrium help - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-22-2011, 11:24 AM
Pipette Filler
Points: 841, Level: 16 Points: 841, Level: 16 Points: 841, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2010
Location: Belfast
Posts: 8
Thanks: 0
Thanked 1 Time in 1 Post
Default sedimentation equilibrium help



Hi I don't know wether or not this is the right place to post but we shall see.

I am looking to see if a drug I work with interacts with proteins that my lab has an interest in. Our initial plan was to use size exclsuion chromatography of protein mixed with drug and then measure the fluorescence of the flow-thru, however we found that the drug bound to the column so this ruled out this option.

We are now looking to carry out sedimentation equilibrium experiments; spin a mixture of drug and protein of interest and see if the drug cosediments with the protein. The only problem is I have no idea how to carry out any sort of experiment using an ultracentrifuge! Would anyone have any good protocols or advice to get me started?

Regards
Wandrews
Reply With Quote
Reply

Tags
equilibrium , sedimentation


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
help in the calculation of the equilibrium dissociation constant kidd Cell Biology and Cell Culture 0 02-27-2011 08:54 PM
Dextran sedimentation sajopik Protocols and Methods Forum 0 01-28-2010 06:54 PM
Chemistry Thermal Equilibrium problem? Ihatecalculus Chemistry Forum 0 11-03-2008 12:18 AM
Mechanics: Equilibrium problem howa Physics Forum 2 10-28-2008 03:09 AM
Equilibrium constant help me Antonino Chemistry Forum 0 10-02-2003 09:59 AM


All times are GMT. The time now is 11:15 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11160 seconds with 16 queries