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protein concentration decrease after protein purification

protein concentration decrease after protein purification - Protein Science

protein concentration decrease after protein purification - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 03-19-2011, 01:38 PM
Pipette Filler
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Unhappy protein concentration decrease after protein purification



Hi,

I did purification (his tag) by FPCL after that I did dialysis

I need injection for rabbit but a protein concentration decrease to 97 g/ml. it

is not enough For injection

Do you know what I can do?



Thank you
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Old 04-28-2011, 09:03 AM
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Default Re: protein concentration decrease after protein purification

Amicon Centrifugal Filters (Millipore) should help u for concentration
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Old 04-28-2011, 09:21 AM
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Default Re: protein concentration decrease after protein purification

You can also freeze dry/lyophilize the reaction but depends what you want to do with the protein later

Also what dialysis membrane did you use (pore size)? What did you dialyze against?
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