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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


ubiquitination

ubiquitination - Protein Science

ubiquitination - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 10-20-2010, 05:13 AM
Pipette Filler
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Default ubiquitination



hi, can someone help me with this question? thanks!

"In a typical fairy shrimp cell there are only 10 proteins that show up in a western blot using an anti-ubiquitin antibody. you over-express an endopeptidase in fairy shrimp cells and find that 5 additional proteins are ubiquitin-tagged. you later find a mutation that suppresses this effect: in mutant cells overexpressing the endopeptidase, only the typical 10 ubiquitinated proteins are detected. how do endopeptidases regulate ubiquitination and what type of protein is likely missing in the mutant?
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