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function of unknown gene

function of unknown gene - Protein Science

function of unknown gene - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 06-27-2010, 05:16 AM
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Default function of unknown gene



If you have a gene sequence which is known to be expressed but does not have a biochemical or physiological function, then how can one determine the function and prove it, lets say in a plant system? Also consider the fact that you have to work from scratch no database (gene bank, blast,etc) is avaliable???
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Old 06-28-2010, 11:02 PM
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Default Re: function of unknown gene

A couple ways to approach it:
1) Look for homologous genes in a species for which you do have a database available.
2) Overexpress it and look for phenotype changes.
3) Knock it down with siRNA or similar and look for phenotype changes.
4) Cross-link and immunoprecipitate it, then use mass spec to identify proteins it interacts with to determine processes it's involved in.
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Old 06-28-2010, 11:27 PM
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Default Re: function of unknown gene

Thanks a lot for the response. What if the unknown gene is invovled in a biochemical pathway and is responsible for synthesis of some intermediate, or phytochemical,etc...then how will overexpression in this case help, because I think these internal biochemical changes wouldnt be seen in phenotype (plants)....I hope I am thinking in right direction. Thanks !
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Old 08-29-2010, 06:13 AM
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Default Re: function of unknown gene

Why no database is available? It is a strange constrain.
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