I need some opinions from you.
I have a protein that forms trimers, dimers, and monomers.
In a SDS-Gel I denature everything and in a Western I just see 1 band.
In a Native Gel, I just can see a monomer...
So, basically, I need a way to separate this forms without denaturate them. (and a cheap metod to do it)
I already try some size exclusin columns. But, in this kind of columns, we are unable to recover the protein that binds to the membrane. We just recover the protein above the cut off