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Small polypeptide in Baculovirus system

Small polypeptide in Baculovirus system - Protein Science

Small polypeptide in Baculovirus system - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 11-13-2009, 01:44 AM
Pipette Filler
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Default Small polypeptide in Baculovirus system



Hi,

I'm trying to purify a recombinant polypeptide (43 residues long) using the baculovirus expression system. I've been successful with this system isolating and analyzing full size protein (60-90KD) but this is my first attempt using the system for a polypeptide.

First, is a polypeptide this small possible to construct + isolate using the baculovirus expression system?

Second, anyone know any literature or have experience that can offer advice?

Third, If this isn't possible, whats the best way to produce a polypeptide this small, synthetically? using another system?

Thanks in advance.
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baculovirus , polypeptide , protein expression , recombinant protein , small , system


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