I'm trying to purify a recombinant polypeptide (43 residues long) using the baculovirus expression system. I've been successful with this system isolating and analyzing full size protein (60-90KD) but this is my first attempt using the system for a polypeptide.
First, is a polypeptide this small possible to construct + isolate using the baculovirus expression system?
Second, anyone know any literature or have experience that can offer advice?
Third, If this isn't possible, whats the best way to produce a polypeptide this small, synthetically? using another system?
Thanks in advance.