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| Hi, I'm trying to purify a recombinant polypeptide (43 residues long) using the baculovirus expression system. I've been successful with this system isolating and analyzing full size protein (60-90KD) but this is my first attempt using the system for a polypeptide. First, is a polypeptide this small possible to construct + isolate using the baculovirus expression system? Second, anyone know any literature or have experience that can offer advice? Third, If this isn't possible, whats the best way to produce a polypeptide this small, synthetically? using another system? Thanks in advance. |
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| baculovirus , polypeptide , protein expression , recombinant protein , small , system |
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