removing cells without damaging the ECM

[Joaquin]

Hi,

I would like to study extracellular matrix secreted by cultured cells. The problem is how to remove the cells but still keep the ECM as intact as possible. After removing the cells I would like to plate new cells and study their adhesion etc. on to the plate.

If someone knows an articel or has experience on the matter I would appreciate the help.

[MatthewDooley]

How is it possible? I don't think that it is possible that removing cells without damaging the ECM. I've not any idea for it. If you have to know any about it than please post it here.

[biolog]

Hi Joaquin,
I have the same problem, and therefore would like to know, if somebody found a solution to the problem of removing cells without destroying the ECM?
thanks

[biolog]

This guy seems to have found a way:

Visualizing Cell Extracellular Matrix (ECM) Deposited by Cells Cultured on Aligned Bacteriophage M13 Thin Films


Laying Wu†‡, L. Andrew Lee†, Zhongwei Niu§, Soumitra Ghoshroy‡, and Qian Wang*†
Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, Columbia, South Carolina 29208, United States
Electron Microscopy Center, University of South Carolina, Columbia, South Carolina 29208, United States
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Langmuir, 2011, 27 (15), pp 9490–9496
DOI: 10.1021/la201580v
Publication Date (Web): June 16, 2011
Copyright © 2011 American Chemical Societ

[biolog]

These guys seem to have found a solution, though.

Visualizing Cell Extracellular Matrix (ECM) Deposited by Cells Cultured on Aligned Bacteriophage M13 Thin Films


Laying Wu†‡, L. Andrew Lee†, Zhongwei Niu§, Soumitra Ghoshroy‡, and Qian Wang*†
Langmuir, 2011, 27 (15), pp 9490–9496
DOI: 10.1021/la201580v
Publication Date (Web): June 16, 2011
Copyright © 2011 American Chemical Society