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PAGE gel storage and choice of loading control

PAGE gel storage and choice of loading control - Protein Science

PAGE gel storage and choice of loading control - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 10-14-2009, 10:58 AM
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Default PAGE gel storage and choice of loading control



Hello,

1. Once the PAGE is prepared, how long can it be stored and under what conditions? For instance, if I prepare the gel on Friday afternoon, can I put the set gel in tank buffer and then store it in the fridge till Monday? Or would simply wrapping the gels in mQ H2O soaked paper towels work just as well?

2. I'm having trouble with our Santa Cruz alpha-tubulin antibody (SC-8035). It doesn't work well---its temperamental, sometimes it works and other times not and we don't know why. Anyone else having these problems? Found a solution? Alternatively, can one suggest another anti-house-keeping antibody which can be used as a loading control but also has multispecies activity. Ideally, I'm looking for something active against rat, goldfish and zebrafish. An alternative alpha-tubulin antibody would be most welcome.

p38 has been suggested and this would be great as my target proteins are all much larger than 38kDa but does it have multispecies activity?

Thanks
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