I'm working with glycoprotein and have 2 glycosylation sites. i mutated one and other one I'm trying to get rid by digesting it with Endo H. I did small scale and it worked fine but when I did large scale i still see not fully deglycosylated band on my gel. Somehow the the protein is not getting fully deglycosylated, i tried using 10 fold more enzyme + more time still no change. How can I fully deglycosylate my protein since little bit of heterogenity will interfere with my crystal structure. Plz let me know!