Hey guys and gals
The problem is that while the proteins belong to the same class, they're from different families, so it's not like there's just a point mutation that will separate them and there's insufficient data available in the literature regarding thermal/chemical denaturing for me to evaluate all the sequence side by side.
Thus, i'm hoping to find at least some sort of digital means to help me compare all the sequences that i've found and then i'm going to compare at least a few that i could find in the literature against the output
i'm interested in comparing proteins from the same class for stability/folding and expression - basically, i'd like to pick the most stable one that would likely be expressed well in Ecoli. any thoughts or suggestions?
I was going to use propparam from expasy and ProSo, but am open to more suggestions and would love to hear what you have to say