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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


protein estimation

protein estimation - Protein Science

protein estimation - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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  #1  
Old 03-13-2009, 09:21 AM
Pipette Filler
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Default protein estimation



respected there
how can we estimate proteins by lowray and bradeford methods in laboratory
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Old 03-13-2009, 01:17 PM
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Default Re: protein estimation

Try a protein assay kit.
Like Pierce BCA Protein Assay --> [Only registered users see links. ]
GE healthcare and Bio-Rad make similar kits as well.
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Old 06-10-2010, 12:22 PM
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Default Re: protein estimation

Well, In my opinion The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained.
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