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| Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below. |
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#1
03-13-2009, 09:21 AM
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 protein estimation respected there how can we estimate proteins by lowray and bradeford methods in laboratory   |
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#2
03-13-2009, 01:17 PM
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| Re: protein estimation Try a protein assay kit. Like Pierce BCA Protein Assay --> [Only registered users see links. ] GE healthcare and Bio-Rad make similar kits as well. |
| The Following User Says Thank You to danfive For This Useful Post: | ||
admin (03-13-2009)
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#3
06-10-2010, 12:22 PM
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| Re: protein estimation Well, In my opinion The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. |
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