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-   -   protein estimation (http://www.molecularstation.com/forum/protein-science/65154-protein-estimation.html)

sadhana singh 03-13-2009 09:21 AM

protein estimation
 
respected there
how can we estimate proteins by lowray and bradeford methods in laboratory:cry:

danfive 03-13-2009 01:17 PM

Re: protein estimation
 
Try a protein assay kit.
Like Pierce BCA Protein Assay --> [Only registered and activated users can see links. Click Here To Register...]
GE healthcare and Bio-Rad make similar kits as well.

mrksmth786 06-10-2010 12:22 PM

Re: protein estimation
 
Well, In my opinion The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained.


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