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· · · Protein Pictures 16 photos 9 comments |
· · · Protein Pictures 16 photos 9 comments |
· · · Protein Pictures 16 photos 9 comments | |||
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| I have always found the checkerboard titration to be a good and fast way to optimize your elisa. Not only does it optimize 3 factors at one go but it gives you a good and clear picture of what dilutions and concentration to use for your desired readings...
__________________ MicroKiller Think out of the box!! |
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| use dot blot (known ag conc) and first standardize the antibody dilution . once u know the best ag-ab combination, u can try different blocking reagents, inacse u see a high background level. the best way to reduce background is to use 1% detergent. incubate o/n with primary ab wash thoroughly (4X) after conjugated secondary ab and develop the ELISA hope this helps. ruby |
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