Sensitivity Close to ECL Detection for Proteins?

nilaanjaana · #1 (**permalink**) 07-29-2008, 10:29 AM

Hi

I want to do western blotting. Is there any detection method that is as sensitive as ECL?

kevin_a · #2 (**permalink**) 07-29-2008, 03:52 PM

Depends on what you mean by ECL.

Some ECL substrates allow you to have picogram levels of detection, others will give you femtogram (mid to low attamoles) levels of detection. For the high pico gram levels you can use fluorescence detection. For the attamole levels you would use 32p or 35s.
If you only need micro and nanogram levels you can use a color precipitating substrate.

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Sensitivity Close to ECL Detection for Proteins?

Sensitivity Close to ECL Detection for Proteins? - Protein Science

Sensitivity Close to ECL Detection for Proteins? - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.