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#1
07-29-2008, 09:29 AM
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 Sensitivity Close to ECL Detection for Proteins? Hi I want to do western blotting. Is there any detection method that is as sensitive as ECL?  |
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#2
07-29-2008, 02:52 PM
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 Re: Sensitivity Close to ECL Detection for Proteins? Depends on what you mean by ECL. Some ECL substrates allow you to have picogram levels of detection, others will give you femtogram (mid to low attamoles) levels of detection. For the high pico gram levels you can use fluorescence detection. For the attamole levels you would use 32p or 35s. If you only need micro and nanogram levels you can use a color precipitating substrate. |
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| close , detection , ecl , proteins , sensitivity |
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